Here’s the grades thus far and some remarks:
Question 1:
Layal :
4.25 out of 5.
“Indicate” doesn’t require justification, neither does “Draw out” (questions 1.1 and 1.6)
Nitrogenous base is not the same as nucleotide. (question 1.3)
Pay attention to 1.5, you have to rule out the heterozygous genotype.
Tala: 4.75 out of 5
“Draw out” doesn’t require justification
the restriction enzyme recognizes the restriction site, not the other way around.
Mounir
2 out of 5
1.1 refer to individuals in the pedigree by their numbers
1.2 “Indicate” doesn’t require justification, don’t write the genotypes on chromosomes that look like the X chromosome, the gene is autosomal as said in the given.
1.3 Pinpoint the difference between the two sequences, don’t just say the 2 alleles have different sequence!
1.4 You messed the whole thing up, you’ll need to review the concept of restriction fragments.
1.5 You erred here too since you didn’t understand the correspondence between the bands and the alleles.
Aya:
3.25 out of 5
1.1 The normal allele masks the morbid allele in the parents, hence the morbid allele is recessive.
1.4 you need to pay closer attention to the given and to review the concept of RFLP analysis
1.5 you lost 0.75 point because of the confusion you had in 1.4
Bassel
4.5 out of 5
1.1 The normal allele masks the morbid allele in the parents, hence the morbid allele is recessive.
1.2 “Indicate” doesn’t require justification
1.3 Minor counting mistake costs you -0.25
1.6 “Draw out” doesn’t require justification
Batoul
4 out of 5 on question 1
1.2 you wasted a lot of time proving that the disease is autosomal. It was GIVEN in the very first line!
1.3 Nitrogenous base is not the same as nucleotide.
1.4 you forgot to determine the restriction fragments of allele d and thus lost -0.75 point
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.1 “Draw out” doesn’t require justification
Leen
4.5 out of 5
1.1 refer to individuals in the pedigree by their numbers
1.3 Nitrogenous base is not the same as nucleotide.
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.6 “Draw out” doesn’t require justification
Sara Kanso
4 out of 5
1.1 The normal allele masks the morbid allele in the parents, hence the morbid allele is recessive.
1.2 “Indicate” doesn’t require justification
1.4 you skipped the morbid allele! Lost half the grade on that question.
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
Maram
3.25 out of 5
1.1 refer to individuals in the pedigree by their numbers\
1.2 you forgot the heterozygote genotype. Genotypes should not be enclosed in square brackets
1.4 the size of each fragment was miscalculated and the justification was incomplete.
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.6 “Draw out” doesn’t require justification
Majd
4 out of 5
1.1 refer to individuals in the pedigree by their numbers\
1.2 “Indicate” doesn’t require justification
1.3 93rd nucleotide, not 91st.
1.5 you discussed I1 instead of II1, pay attention next time.
1.6 “Draw out” doesn’t require justification
Maya
4.5 out of 5
1.1 The normal allele masks the morbid allele in the parents, hence the morbid allele is recessive. 1.2 “Indicate” doesn’t require justification 1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype. 1.6 “Draw out” doesn’t require justification
Fares
4.25 out of 5
1.1 refer to individuals in the pedigree by their numbers\
1.2 “Indicate” doesn’t require justification, genotype of II1 is dd, not NN
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.6 “Draw out” doesn’t require justification
Ibtissam
4.75 out of 5
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.6 “Draw out” doesn’t require justification
Nadine:
3 out of 5
1.2 the gene is autosomal as said in the given.
1.3 specify the location of the mutated nucleotide.
1.4 you skipped the morbid allele! Lost half the grade on that question.
1.6 “Draw out” doesn’t require justification
Nour
5 out of 5
Ali
2.25 out of 5
1.1 refer to individuals in the pedigree by their numbers\
1.2 “Indicate” doesn’t require justification.
1.4 the number and size of restriction fragments was miscalculated.
1.5 erred here as a consequence of your error in 1.4
Sami
2 out of 5
1.1 refer to individuals in the pedigree by their numbers\
1.2 “Indicate” doesn’t require justification.
1.3 reference the mutation correctly (93rd nucleotide) and say that it’s a substitution.
1.4 Completely wrong
1.5 Affected by your error in 1.4
Khachfe
4 out of 5
1.4 you forgot to calculate the sizes of the fragments and thus lost 0.75 point
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.6 “Draw out” doesn’t require justification
Ghiwa
2 out of 5
1.2 the healthy individual II1 could also be heterozygote Nd
1.3 specify the number of affected nucleotide and name it ‘substitution
1.4 you need to review the concept of RFLP analysis.
1.5 You couldn’t relate the pattern of bands to the corresponding allele.
1.6 again, you lost the grade for not being able to correlate the electrophoregrams to the genotypes.
Hariri
3.75 out of 5
1.2 “Indicate” doesn’t require justification.
1.3 93rd nucleotide and not 7th codon
1.4 you skipped the morbid allele! Lost half the grade on that question.
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.6 “Draw out” doesn’t require justification
Sheaib
3.25 out of 5
1.1 refer to individuals in the pedigree by their numbers\
1.2 “Indicate” doesn’t require justification.
1.3 reference the mutation correctly (93rd nucleotide).
1.4 you skipped the morbid allele! Lost half the grade on that question.
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
Hamza
5 out of 5
1.6 “Draw out” doesn’t require justification
Rayan
4.5 out of 5
1.1 The normal allele masks the morbid allele in the parents, hence the morbid allele is recessive.
1.5 Pay attention to 1.5, you have to rule out the heterozygous genotype.
1.6 “Draw out” doesn’t require justification
Sara Alameh
2.75 out of 5
1.1 refer to individuals in the pedigree by their numbers
1.2 “Indicate” doesn’t require justification.
1.3 Substitution is not a frameshift-causing mutation.
1.4 You did not calculate the size of fragments, plz review RFLP analysis
1.5 Incomplete justification
I’ll stop updating this stream since I’ll hopefully deliver the results in person tomorrow morning.