Introduction to Pedigree Symbols and analysis

Pedigree symbols Males are represented by squares. Females are represented by circles. Filled symbols show individuals who exhibit the trait in question. A horizontal line between two symbols represents a mating. A vertical line descends from parents to a horizontal line shared by all their offspring. A diagonal line through a symbol indicates that person …

FISH conceptual drawing

So you have this gene whose nucleotide sequence is known to you but its chromosomal location (locus) is unknown, how do you go about finding this locus? The short answer is FISH i.e fluorescence in situ hybridization. Here’s a quick list of the steps of FISH technique: 1- prepare a fluorescent probe (short nucleotide sequence …